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| Registros recuperados: 35 | |
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| Rolland, Jean-luc; Medhioub, Walid; Vergnes, Agnes; Abi-khalil, Celina; Savar, Veronique; Abadie, Eric; Masseret, Estelle; Amzil, Zouher; Laabir, Mohamed. |
| To better understand the effect of Paralytic Shellfish Toxins (PSTs) accumulation in the digestive gland of the Pacific oyster, Crassostrea gigas, we experimentally exposed individual oysters for 48 h to a PSTs producer, the dinoflagellate Alexandrium catenella. In comparison to the effect of the non-toxic Alexandrium tamarense, on the eight apoptotic related genes tested, Bax and BI.1 were significantly upregulated in oysters exposed 48 h to A. catenella. Among the five detoxification related genes tested, the expression of cytochrome P450 (CYP1A) was shown to be correlated with toxin concentration in the digestive gland of oysters exposed to the toxic dinoflagellate. Beside this, we observed a significant increase in ROS production, a decrease in... |
| Tipo: Text |
Palavras-chave: Shellfish; Toxin; Biomarker; Expression; Phytoplankton. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00225/33606/32003.pdf |
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| Assis,Helena Cristina da Silva de; Nicareta,Lilian; Salvo,Ligia Maria; Klemz,Claudio; Truppel,Jessé Henrique; Calegari,Rossana. |
| This study aimed to determine the effect of sublethal doses of deltamethrin, using biochemical biomarkers as activities of cholinesterase (ChE), ethoxyresorufin-O-deethylase (EROD) and the Na+K+- ATPase and levels of total cytochrome P450 (CYP450). Fishes received sublethal doses of deltamethrin and were sacrificed after 96 h of exposure. Samples of gills, heart, brain, liver and muscle were collected for enzymatic analyses. Deltamethrin inhibited the activity of the gills and heart Na+ K+-ATPase, induced the liver total CYP450, as well as the liver EROD activity. The activity of the ChE was not inhibited by deltamethrin. Deltamethrin altered the hepatic metabolism and the normal ionic flux in Ancistrus multispinis. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Deltamethrin; Cytochrome P450; Fish; Ancistrus multispinis; Biomarker. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000600012 |
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| Kong,X.D.; Liu,N.; Xu,X.J.. |
| In this study, biomarkers and transcriptional factor motifs were identified in order to investigate the etiology and phenotypic severity of Down syndrome. GSE 1281, GSE 1611, and GSE 5390 were downloaded from the gene expression ominibus (GEO). A robust multiarray analysis (RMA) algorithm was applied to detect differentially expressed genes (DEGs). In order to screen for biological pathways and to interrogate the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway database, the database for annotation, visualization, and integrated discovery (DAVID) was used to carry out a gene ontology (GO) function enrichment for DEGs. Finally, a transcriptional regulatory network was constructed, and a hypergeometric distribution test was applied to select for... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Down syndrome; Candidate gene; Biomarker; Transcriptional factor motifs. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014001000834 |
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| Xu,Li; Peng,Hui; Huang,Xiao-Xu; Xia,Ya-Bin; Hu,Kai-Feng; Zhang,Zheng-Ming. |
| Previous studies suggested that chromodomain helicase DNA-binding proteins (CHDs), including CHD 1–8, were associated with several human diseases and cancers including lymphoma, liver cancer, colorectal cancer, stomach cancer, etc. To date, little research on CHD 9 in human cancers has been reported. In this study, we assessed the prognostic value of CHD 9 in patients with colorectal cancer (CRC). We screened for CHD 9 expression using immunohistochemical analysis in 87 surgical CRC specimens and found that the expression was upregulated in 81.5% of the cases, while 7.4% were decreased; in the remaining 11.1% of the cases, levels were not altered. Kaplan-Meier analysis showed that patients with high CHD 9 expression had better prognosis than those with low... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chromodomain helicase DNA-binding protein 9; Colorectal cancer; Immunohistochemical analysis; Biomarker; Prognosis. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000900612 |
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| Rangel,M.P.; Antonangelo,L.; Acencio,M.M.P.; Faria,C.S.; de Sá,V.K.; Leão,P.S.; Farhat,C.; Fabro,A.T.; Longatto Filho,A.; Reis,R.M.; Takagaki,T.; Capelozzi,V.L.. |
| Cofilin-1 (CFL1), a small protein of 18 kDa, has been studied as a biomarker due to its involvement in tumor cell migration and invasion. Our aim was to evaluate CFL1 as an indicator of malignancy and aggressiveness in sputum samples. CFL1 was analyzed by ELISA immunoassay in the sputum of 73 lung cancer patients, 13 cancer-free patients, and 6 healthy volunteers. Statistical analyses included ANOVA, ROC curves, Spearman correlation, and logistic regression. Sputum CFL1 levels were increased in cancer patients compared to cancer-free patients and volunteers (P<0.05). High expression of sputum CFL1 was correlated to T4 stage (P=0.01) and N stage (P=0.03), tobacco history (P=0.01), and squamous cell carcinoma histologic type (P=0.04). The accuracy of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Lung cancer; Cofilin-1; Sputum; Liquid-biopsy; Metastases; Biomarker. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000800603 |
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| Rees, Jg; Setiapermana, D; Sharp, Va; Weeks, Jm; Williams, Tm. |
| To evaluate the impact of land-based contaminants on benthic faunas, as part of the Land-Ocean Contamination Study (LOCS), a study transect was established in Jakarta Bay, Indonesia during the 1996 southeasterly monsoon. The transect extended 72 km between the city's main port, Tanjung Priok, and the Pilau Seribu Island chain in the Java Sea. The dissolved concentrations of Pb, Cu, Zn, Cr and Ni in seawater, in suspended particulate matter (SPM) and surficial sea bed sediments were measured along the transect at nine sites. In addition, metal concentrations were measured in tissues of the corals Goniopora lobata and Lobophyllia hemprichii at five sites, and of the green mussel, Perna viridis at six sites. An assessment of the impact of contaminants on the... |
| Tipo: Text |
Palavras-chave: Djakarta; Contamination; Biomarqueur; Communauté corallienne; Jakarta; Contamination; Biomarker; Coral-community. |
| Ano: 1999 |
URL: http://archimer.ifremer.fr/doc/00325/43588/44028.pdf |
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| Sumrin,Aleena; Moazzam,Shumaila; Khan,Aleena Ahmad; Ramzan,Irsa; Batool,Zunaira; Kaleem,Sana; Ali,Moazzam; Bashir,Hamid; Bilal,Muhammad. |
| ABSTRACT Rapid advances in medicine and biotechnology resulted in the development of non-invasive diagnostic and prognostic biomarkers enabling convenient and accurate detection. Exosomes has recently emerged as non-invasive biomarker for a number of diseases including cancer. Exosomes are the small endosome originated membranous vesicles secreted in a number of biological fluids such as serum, saliva, urine, ascites, cerebrospinal fluid, etc. Exosomes contain microRNA proteins and mRNA which can be used as disease specific biomarkers. Here we reviewed recent advancement in the field of exosomes as diagnostic biomarker for cancer along with a brief overview of their biogenesis, function and isolation. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Exosome; Biomarker; Cancer biomarker. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100309 |
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| Arámbula-Meraz,Eliakym; Bergez-Hernández,Fernando; Leal-León,Emir; Romo-Martínez,Enrique; Picos-Cárdenas,Verónica; Luque-Ortega,Fred; Romero-Quintana,Jose; Alvarez-Arrazola,Marco; García-Magallanes,Noemí. |
| Abstract Prostate cancer (PCa) is one of the leading causes of death among men. Genes such as PCA3, PSA, and Fra-1 are suggested to serve as potential tools for the detection of PCa, as they are deregulated during this pathology. A similar event occurs with small non-coding RNAs, called miRNAs, specifically miR-195-5p, miR-133a-3p, and miR-148b-3p, which were analyzed in a Chinese population and suggested to be possible candidates for PCa diagnosis. We evaluated the expression levels of three miRNAs and three genes in tissue samples of PCa and benign prostate disease, such as benign prostatic hyperplasia, or prostatitis, in order to determine their potential as candidates for PCa detection. Our results showed a statistically significant overexpression of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Gene expression; Biomarker; MiRNAs; Correlation; Prostate cancer. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100112 |
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| Chatel, Annelle; Faucet-marquis, Virginie; Gourlay-france, Catherine; Pfohl-leszkowicz, Annie; Hubert, Francoise. |
| The aim of the present study was to confirm the relevance of studying DNA adduct formation in a field study. In that context, freshwater mussels Dreissena polymorpha, collected in a reference station, were transplanted in different sites with a pollution gradient. After one and two months, mussels were collected and DNA adduct formation was analyzed using the 32P post labelling technique on both gills and digestive glands. In addition, the expression of genes involved in the detoxification system (catalase (CAT), superoxide dismutase (SOD), glutathione S-transferase (GST), HSP70, aryl hydrocarbon receptor (AHR), P glycoprotein (PgP), metallothionein (MT)) was assessed by RT-PCR. DNA adducts were observed at amount comparable to data from literature.... |
| Tipo: Text |
Palavras-chave: Biomarker; Pollution; DNA adducts; Detoxification; Dreissena polymorpha; RT-PCR. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00195/30639/32450.pdf |
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| Feng,H.J.; Ouyang,W.; Liu,J.H.; Sun,Y.G.; Hu,R.; Huang,L.H.; Xian,J.L.; Jing,C.F.; Zhou,M.J.. |
| Hypertrophy is a major predictor of progressive heart disease and has an adverse prognosis. MicroRNAs (miRNAs) that accumulate during the course of cardiac hypertrophy may participate in the process. However, the nature of any interaction between a hypertrophy-specific signaling pathway and aberrant expression of miRNAs remains unclear. In this study, Spague Dawley male rats were treated with transverse aortic constriction (TAC) surgery to mimic pathological hypertrophy. Hearts were isolated from TAC and sham operated rats (n=5 for each group at 5, 10, 15, and 20 days after surgery) for miRNA microarray assay. The miRNAs dysexpressed during hypertrophy were further analyzed using a combination of bioinformatics algorithms in order to predict possible... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cardiac hypertrophy; Signaling pathway; Biomarker. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000500361 |
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| Moncau,Cristina Tschorny; Rosa,Alessandra Fernandes; Eler,Joanir Pereira; Balieiro,Júlio César de Carvalho. |
| Abstract: The objective of this work was to quantify heat shock proteins (HSP) 27 and 70 in the Longissimus dorsi muscle of cattle during aging and to check their potential as biomarkers for meat quality. A total of 191 steers ½ South African Simmental x ½ Nellore (16-18 months, 391.7±99.7 kg), castrated, and feedlot finished were used. Meat quality was measured by pH, color, cooking loss, and shear force (SF) at 1 and 14 days of aging time. HSP27 and HSP70 were quantified according to the SF values in the more and less tender meat groups, with 20 samples each, for each aging time. HSP27 concentrations in more and less tender meat decrease from 1 to 14 days of aging, and do not differ when evaluated at the same period. HSP70 concentrations in more tender... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bos taurus indicus; Biomarker; HSP27; HSP70; Meat tenderness. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2018000100090 |
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| Vethaak, A. Dick; Davies, Ian M.; Thain, John E.; Gubbins, Matthew J.; Martinez-gomez, Concepcion; Robinson, Craig D.; Moffat, Colin F.; Burgeot, Thierry; Maes, Thomas; Wosniok, Werner; Giltrap, Michelle; Lang, Thomas; Hylland, Ketil. |
| Many maritime countries in Europe have implemented marine environmental monitoring programmes which include the measurement of chemical contaminants and related biological effects. How best to integrate data obtained in these two types of monitoring into meaningful assessments has been the subject of recent efforts by the International Council for Exploration of the Sea (ICES) Expert Groups. Work within these groups has concentrated on defining a core set of chemical and biological endpoints that can be used across maritime areas, defining confounding factors, supporting parameters and protocols for measurement. The framework comprised markers for concentrations of, exposure to and effects from, contaminants. Most importantly, assessment criteria for... |
| Tipo: Text |
Palavras-chave: Chemical measurements; Biomarker; Bioassay; Pollution effects; Biomonitoring; Environmental; Impact; MSFD; ICES; OSPAR. |
| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00283/39429/37869.pdf |
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| Wang,J.Z.; Xu,C.L.; Wu,H.; Shen,S.J.. |
| Several long non-coding RNA (lncRNA) might be correlated with the prognosis of colorectal cancer (CRC) and serve as a diagnostic and prognostic biomarker. However, the exact expression pattern of small nucleolar RNA host gene 12 (SNHG12) in colorectal cancer and its clinical significance remains unclear. The level of SNHG12 was detected by qRT-PCR in CRC tissues and CRC cells. MTT assay and colony formation assay were performed to examine the cell proliferation of CRC cells transfected with pcDNA-SNHG12 or si-SNHG12. Flow cytometry technology was used to detect cell cycle and cell apoptosis of CRC cells transfected with pcDNA-SNHG12 or si-SNHG12. The protein level of cell cycle progression-related molecules, including cyclin-dependent kinases (CDK4, CDK6),... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: SNHG12; Cell growth; Biomarker; Cell apoptosis; Colorectal cancer. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000300604 |
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| MADEIRA,KRISTIAN; DONDOSSOLA,EDUARDO R.; FARIAS,BRUNA F. DE; SIMON,CARLA S.; ALEXANDRE,MARIA C.M.; SILVA,BRUNO R.; ROSA,MARIA INÊS. |
| The objective of this work was to estimate the accuracy of mesothelin as a biomarker for ovarian cancer. A quantitative systematic review was performed. A comprehensive search of the Medline, LILACS, SCOPUS, Embase, Cochrane Central Register of Controlled Trials, Biomed Central, and ISI Web of Science databases was conducted from January 1990 to June 2015. For inclusion in this systematic review, the papers must have measured mesothelin levels in at least two histological diagnoses; ovarian cancer (borderline or ovarian tumor) vs. benign or normal ovarian tissue. For each study, 2 x 2 contingency tables were constructed. We calculated the sensitivity, specificity and diagnostic odds ratio. The verification bias was performed according to QUADAS-2.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ovarian tumors; Mesothelin; Meta-analysis; Biomarker; Statistical. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000300923 |
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| Registros recuperados: 35 | |
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